The DSC specializes in protocol development & custom sequencing applications. Selected publications that resulted from this work include:

SPAR-Seq

Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors.
Han H, Braunschweig U, Gonatopoulos-Pournatzis T, Weatheritt RJ, Hirsch CL, Ha KC, Radovani E, Nabeel-Shah S, Sterne-Weiler T, Wang J, O’Hanlon D, Pan Q, Ray D, Zheng H, Vizeacoumar F, Datti A, Magomedova L, Cummins CL, Hughes TR, Greenblatt JF, Wrana JL, Moffat J, Blencowe BJ.
 2017. doi: 10.1016/j.molcel.2017.01.011.

LIGR-Seq

Global Mapping of Human RNA-RNA Interactions.
Sharma E, Sterne-Weiler T, O’Hanlon D, Blencowe BJ.
Molecular Cell. 2016. doi: 10.1016/j.molcel.2016.04.030.

CRISPR

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens.
Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J.
G3 (Bethesda). 2017. doi: 10.1534/g3.117.041277.

Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z, Pavlovic Z, Chandrashekhar M, Hart T, Wang X, Zhang X, Robitaille M, Brown KR, Jaksani S, Overmeer R, Boj SF, Adams J, Pan J, Clevers H, Sidhu S, Moffat J, Angers S.
Nature Medicine. 2017. doi: 10.1038/nm.4219.

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.
Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J.
Cell 2015. doi: 10.1016/j.cell.2015.11.015.

Phage-display

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